Abstract Objective To investigate the clinical usefulness of serum superoxide dismutase (SOD) measurement in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Methods In this single-center retrospective study, demographic data, serum SOD levels, erythrocyte sedimentation rate (ESR), C reactive protein (CRP), the Birmingham Vasculitis Activity Score (BVAS), ANCA, organ involvement, and outcomes were analyzed for 152 AAV patients hospitalized in the Second Affiliated Hospital of Chongqing Medical University. Meanwhile, the serum SOD levels of 150 healthy people were collected as the control group. Results Compared to the healthy control group, serum SOD levels of the AAV group were significantly lower (P < 0.001). SOD levels of AAV patients were negatively correlated to ESR, CRP, and BVAS (ESR rho = − 0.367, P < 0.001; CRP rho = − 0.590, P < 0.001; BVAS rho = − 0.488, P < 0.001). SOD levels for the MPO-ANCA group were significantly lower than the PR3-ANCA group (P = 0.045). SOD levels for the pulmonary involvement group and the renal involvement group were significantly lower than those for the non-pulmonary involvement group and the non-renal involvement group (P = 0.006; P < 0.001, respectively). SOD levels in the death group were significantly lower than the survival group (P = 0.001). Conclusions In AAV patients, low SOD levels might indicate disease associated oxidative stress. SOD levels in AAV patients were decreased with inflammation, suggesting that SOD levels could potentially be a surrogate marker for disease activity. SOD levels in AAV patients were closely related to ANCA serology, pulmonary involvement, and renal involvement, with low SOD levels an important indicator of a poor prognosis for AAV patients.

Abstract The higher acidity in fruits compared with grapes was one of the main obstacles to producing high-quality fruit wines. In the present research, the effect of the split batch fermentation pattern on the metabolites of greengage wines was investigated. This fermentation pattern included whole fruit immersion in the sucrose and coculturing Saccharomyces bayanus Y4 with Torulaspora delbrueckii Y7. These results showed that the contents of total acidity and dominant organic acids were significantly reduced among the batches. Aliphatic compounds were decreased with the batches, while aromatic compounds that contributed to flavor improvement were increased oppositely. The acetate esters, ketones, and terpenes contents were significantly increased in the first batch of coculture. In addition, acidic stress largely affected the kinetics characteristics of greengage wine on a pilot scale. Interestingly, the increment of total acidity was closely associated with the rate of total sugar consumption, which was affected by microbial activities. The acidity content was significantly declined and distinct flavour profiles were formed in split batch and coculture fermentation of greengage wines. It provides a new avenue to enhance the flavor and quality of fruit wines. highquality high research investigated Y Y7 batches oppositely esters ketones addition scale Interestingly consumption activities

RESUMO Objetivo: Analisar a realidade do desenvolvimento do estilo de vida ativo nacional, esclarecer suas limitações e contramedidas. Metodologia: Foram empregados dados oficiais e consultas em referências bibliográficas atualizadas para o levantamento dos principais problemas com o estilo de vida sedentário e suas possíveis soluções. Resultados: Os três problemas urgentes a serem resolvidos nos serviços públicos são a construção de instalações esportivas, organização de atividades esportivas regulares e o estabelecimento de organizações esportivas. Apenas alguns poucos esportes foram promovidos em operações concretas. A educação física inadequada e os serviços públicos essenciais deficitários também são fatores que afetam a saúde nacional. Conclusão: A construção de instalações esportivas e o fornecimento de ambientes com equipamentos esportivos não são suficientes contra o sedentarismo. Políticas relevantes devem ser executadas juntamente com uma propaganda massiva sobre os benefícios do estilo de vida ativo. A orientação contínua de assistentes sociais em grupos esportivos comunitários para promover a participação das massas também é fundamental para a promoção da saúde através de intervenções esportivas.

ABSTRACT Objective: To analyze the reality of the development of the national active lifestyle, clarify its limitations and countermeasures. Methodology: Official data and consultations in updated bibliographic references were employed for the survey of the main problems with a sedentary lifestyle and their possible solutions. Results: The three urgent problems to be solved in public services are the construction of sports facilities, the organization of regular sports activities, and the establishment of sports organizations. Only a few sports have been promoted in concrete operations. Inadequate physical education and deficient essential public services are also factors affecting national health. Conclusion: Building sports facilities and providing environments with sports equipment are not sufficient against sedentary lifestyles. Relevant policies must be implemented along with massive advertising about the benefits of active lifestyles. Continuous orientation of social workers in community sports groups to promote mass participation is also key to health promotion through sports interventions.

RESUMEN Objetivo: Analizar la realidad del desarrollo del estilo de vida activo nacional, aclarar sus limitaciones y contramedidas. Metodología: Para el estudio de los principales problemas del sedentarismo y sus posibles soluciones se emplearon datos oficiales y consultas en referencias bibliográficas actualizadas. Resultados: Los tres problemas urgentes que hay que resolver en los servicios públicos son la construcción de instalaciones deportivas, la organización de actividades deportivas regulares y la creación de organizaciones deportivas. Sólo unos pocos deportes fueron promovidos en operaciones concretas. Una educación física inadecuada y unos servicios públicos esenciales deficientes son también factores que afectan a la salud nacional. Conclusión: La construcción de instalaciones deportivas y la dotación de entornos con equipamiento deportivo no son suficientes contra el sedentarismo. Deben ejecutarse políticas pertinentes junto con una publicidad masiva sobre los beneficios del estilo de vida activo. La orientación continua de los trabajadores sociales en los grupos deportivos comunitarios para promover la participación masiva también es fundamental para la promoción de la salud a través de las intervenciones deportivas.

OBJECTIVES: Inflammatory factors exert a significant role in the development of adult-onset Still’s disease (AOSD) and sepsis. Although platelet counts and platelet parameters have long served as indicators for inflammatory diseases, their role in the differential diagnosis between adult-onset stilĺs disease and sepsis remains unclear. We designed this retrospective study to explore whether the platelet to mean platelet volume (MPV) ratio (PMR) can help to distinguish AOSD from sepsis. METHODS: A total of 110 AOSD patients and 84 sepsis patients were enrolled in the study. Seventy-three AOSD patients and 56 sepsis patients between January 2010 and June 2017 were enrolled in the test cohort to analyze PMR values, which was then validated in the validation cohort (37 AOSD patients and 28 sepsis patients between June 2017 and December 2019). RESULTS: The values of PMR were significantly higher in AOSD patients than in sepsis patients (test cohort, validation cohort, and entire cohort), In the test cohort, logistic regression analysis showed that PMR was an independent risk factor of AOSD (odds ratios [OR]: 9.22, 95% confidence interval [CI] 2.15-39.46, p=0.003). Further receiver operating characteristic curve (ROC) analysis showed that the area under the ROC curve was 0.735 (95% CI 0.631-0.839, p<0.001) for PMR alone and 0.925 (95% CI 0.869-0.980, p<0.001) for the combination of PMR and serum ferritin. Consistently, the validation cohort exhibited analogous results. CONCLUSIONS: PMR could be used as a single indicator or a complementary indicator to distinguish AOSD from sepsis.

RESUMO: A biodiversidade desempenha um papel fundamental no bem-estar humano, proporcionando benefícios agrícolas, econômicos e de saúde. No entanto, após a revolução industrial, a rápida expansão da população humana e as subsequentes atividades econômicas provocaram uma perda drástica na biodiversidade global, resultando em perturbações significativas para os ecossistemas e nossas próprias condições de vida. Consequentemente, a conservação da biodiversidade tornou-se um dos desafios mais para a humanidade. O grande número de plantas, animais e microrganismos; a enorme diversidade genética destas espécies; e os diferentes ecossistemas aos quais pertencem esses organismos fazem parte de um planeta biologicamente diversificado. Uma proporção substancial da biodiversidade mundial foi destruída. Essa perda é uma catástrofe para todas as espécies vivas, incluindo os humanos. Felizmente, estamos trabalhando para remediar a destruição de nossos ecossistemas. Neste artigo resumimos a descoberta e o desenvolvimento da biodiversidade como área de estudo e discutimos a importância da análise funcional e metabólica. Propomos soluções potenciais para a perda de biodiversidade com o objetivo de facilitar a exploração e identificação da biodiversidade, contribuindo para o bem-estar da humanidade através da conservação dos habitats da nossa espécie.

ABSTRACT: Biodiversity plays a key role in human welfare by providing agricultural, economic, and health benefits. However, following the industrial revolution, the rapid expansion of the human population and subsequent economic activities have caused a dramatic loss in global biodiversity, resulting in significant disturbances to ecosystems and our own living conditions. Accordingly, the conservation of biodiversity has become one of the most important challenges for humanity. The vast numbers of plants, animals, and microorganisms, the enormous genetic diversity of these species and the different ecosystems to which these organisms belong are all part of a biologically diverse planet. A substantial proportion of the world’s biodiversity has been destroyed, this loss is a catastrophe for all living species, including humans. Fortunately, we are working to remedy the destruction of our ecosystems. Herein, we summarized the discovery and development of biodiversity as a field of study and discuss the importance of the genetic and metabolite diversity. We proposed potential solutions to the loss of biodiversity with the aim of facilitating further exploration and identification of biodiversity, contributing for human welfare through the conservation of human habitats.

RESUMEN El margen oriental del altiplano Qinghai-Tíbet (QTP, del inglés Qinghai-Tibet Plateau) es el área de la extrusión lateral hacia el Este de material cortical. Este trabajo se enfoca en explorar la respuesta estructural de las capas superiores en el altiplano y las bases para su estructuracón geológica. Se utilizó información magnetotelúria y anomalías de Bouguer para determinar la respuesta geofísica de las capas superiores en el margen occidental del altiplano. Dentro de los principales resultados se tiene: (i) la distribución de la densidad aparente indica la presencia de material de baja densidad en las capas medias y bajas de los bloques Songpan y Sichuan-Yunnan en el Este del QTP. Por otro lado, el bloque cratónico Yangtze (en la cuenca Sichuan) contiene material con una mayor densidad. Al oeste de las zonas tectónicas Longmeshan-Panxi, y a lo largo de las capas inferiores, entre 40 y 50 kilómetros de profundidad, hay una zona de baja densidad con orientación noreste-suroeste. (ii) El modelo eléctrico que abarca el bloque Songpan, la zona tectónica Longmeshan y el bloque Yangtze, revela tres unidades a lo largo de la sección cruzada subordinada a la zona tectónica Longmenshan. La primera unidad está en el bloque Songpan, con alta resistividad en la capa superficial y baja en las capas media e inferiores. Luego aparece el cratón Yangtze, con baja resistividad en la superficie y resistividad media en las capas media e inferiores. La tercera unidad es la zona tectónica transicional de Longmenshan, cuya estructura superficial y profunda está caracterizada por una estructura eléctrica asociada a una falla de cabalgamiento hacia el Este y alta conductividad de material que se extiende hacia el manto litosférico. (iii) La densidad aparente y las estructuras eléctricas indican que la zona tectónica de Panxi está debilitada en las capas inferiores. (iv) las propiedades geofísicas de la estructura profunda del altiplano Qinghai-Tíbet muestran que su margen oriental puede contener un fluido de material en las capas bajas y medias con características de alta conductividad y baja densidad. Su distribución está interrelacionada con el mecanismo de elevación y las actividades sismogénicas profundas en el margen oriental del altiplano.

ABSTRACT The eastern margin of the Qinghai-Tibet Plateau (QTP) is the focus of studies on eastward lateral extrusion of the latter's crustal material. This study aims to explore the structural response of the QTP's eastern crust-mantle to the extrusion, and the basis for the latter's geological structure. Data on the long-period magneto telluric sounding of cross-tectonic units and Bouguer gravity were used to determine the physical structure of the crust-mantle at the plateau's eastern margin. The findings are as follows: (i) the apparent density structure indicates the large presence of a low-density material in the middle-lower crusts of the Songpan and Sichuan-Yunnan blocks at the QTP's eastern margin. On the other hand, the Yangtze cratonic block (Sichuan Basin) contains a material with a significantly higher density. To the west of the Longmenshan-Panxi tectonic zones, and along the lower crust at 40-50 km depth, is an obvious low-density zone aligned in a northeast-southwest orientation. (ii) The electrical structural model spanning Songpan block-Longmenshan tectonic zone-Yangtze block reveals three distinct electrical structural units along the cross-section bounded by the Longmenshan tectonic zone. The first is the Songpan block, which has high and low resistivity at the superficial layer and middle-lower crusts, respectively. Next is the Yangtze craton, which has a low and relatively higher resistivity at the superficial layer and middle-lower crusts, respectively. The third is the Longmenshan transitional tectonic zone, whose shallow layer and deep structure are characterized by an electrical structure with a thrust nappe towards the east, and a high-conductivity material extending to the lithospheric mantle, respectively. (iii) The apparent density and electrical structures indicate that the Panxi tectonic zone has a weakened structure in the lower crust; and (iv) physical properties of the QTP's deep structure show that its eastern margin may contain a middle-lower crustal fluid material with the attributes of high conductivity and low density. Its distribution is interrelated to the uplift mechanism and deep seismogenic activities at the QTP's eastern margin.

PURPOSE: To construct a new biomaterial-small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor, and to evaluate the new biomaterials for the reconstruction of abdominal wall defects.METHODS: Thirty six Sprague-Dawley rats were used in the animal experiments and randomly divided into three groups. The new biomaterial was constructed by combining small intestinal submucosa with gelatin hydrogel for basic fibroblast growth factor release. Abdominal wall defects were created in rats, and repaired using the new biomaterials (group B), compared with small intestinal submucosa (group S) and ULTRAPROTM mesh (group P). Six rats in each group were sacrificed at three and eight weeks postoperatively to examine the gross effects, inflammatory responses, collagen deposition and neovascularization.RESULTS: After implantation, mild adhesion was caused in groups B and S. Group B promoted more neovascularization than group S at three weeks after implantation, and induced significantly more amount of collagen deposition and better collagen organization than groups S and P at eight weeks after implantation.CONCLUSION: Small intestinal submucosa coated with gelatin hydrogel incorporating basic fibroblast growth factor could promote better regeneration and remodeling of host tissues for the reconstruction of abdominal wall defects.

Uma nova neolignana benzofurânica, isovalerato de 9-di-hidrodesidrodiconiferila, além de 10 compostos fenólicos conhecidos, olivil, pinoresinol, 8-hidroxipinoresinol, pinorespiol, 8-hidroxi-7epipinoresinol, ácido trans-p-hidroxifenil-propenoico, ácido cis-p-hidroxifenil-propenoico, ácido isoferúlico e isovanilina foram isolados a partir das raízes da Valeriana officinalis var. latifolia. Suas estruturas e configurações foram elucidadas com base em métodos espectroscópicos. A atividade inibitória da acetilcolinesterase (AChE) e a atividade intensificada do fator de crescimento neural (NGF) mediada pelo crescimento de neurites em células PC12 pelos compostos isovalerato de 9-di-hidrodesidrodiconiferila e olivil foram avaliadas.

A new benzofuran neolignan, dihydrodehydrodiconiferyl alcohol 9-isovalerate, along with ten known phenolic compounds, olivil, pinoresinol, 8-hydroxypinoresinol, pinorespiol, 8-hydroxy7-epipinoresinol, trans-p-hydroxyphenyl- propenoic acid, cis-p-hydroxyphenyl-propenoic acid, ferulic acid, isoferulic acid and isovanillin were isolated from the roots of Valeriana officinalis var. latifolia. Their structures and configurations were elucidated on the basis of spectroscopic methods. The inhibitory activity for acetylcholinesterase (AChE) and enhancing activity on nerve growth factor (NGF)-mediated neurite outgrowth in PC12 cells of dihydrodehydrodiconiferyl alcohol 9-isovaterate and olivil were evaluated.

FUNDAMENTO: O Imatinib é um inibidor do receptor tirosina-quinase que foi confirmada como exercendo um efeito inibidor sobre a atividade do receptor do PDGF, fator de crescimento plaquetário (PDGFRα e PDGFRβ). OBJETIVO: Investigar o efeito protetor do Imatinib na fibrose miocárdica em acetato de deoxicorticosterona (DOCA)/ratos com hipertensão induzida por sal. MÉTODOS: Sessenta ratos Sprague-Dawley machos, uninefrectomizados foram distribuídos em três grupos: ratos controles (grupo CON): grupo deoxicorticosterona (grupo DOCA); grupo deoxicorticosterona e Imatinib (grupo DOCA IMA). A Pressão Arterial Sistólica (PAS) foi medida quinzenalmente. Foi estudada a porção apical do ventrículo esquerdo. Foram empregados: coloração vermelho sirius, coloração de hematoxilina-eosina, imuno-histoquímica e ensaio de western blot. RESULTADOS: A PAS nos grupos DOCA e IMA+DOCA foi maior que no grupo CON nos dias 14 e 28. Os animais do grupo DOCA apresentaram fibrose intersticial e perivascular grave no dia 28, e as expressões de PI, PIII, tenascina-C e fibronectina foram significativamente maiores que nos grupos DOCA+IMA e CON. Quando comparados com o grupo CON, os grupos DOCA e DOCA+IMA apresentaram resposta inflamatória de tecido miocárdico e infiltração de monócitos/macrófagos de diferentes graus. As expressões proteicas do PDGF-A, PDGF-C e PDGFRα foram significativamente maiores nos grupos DOCA e DOCA+IMA que no grupo CON, mas a expressão proteica do p-PDGFRα no grupo DOCA+IMA foi menor que no DOCA. CONCLUSÃO: O Imatinib pode exercer efeitos inibitórios sobre a fibrose miocárdica em ratos com hipertensão induzida por DOCA/sal, os quais podem ser atribuídos à inibição da atividade do PDGFR-α.

BACKGROUND: Imatinib is a tyrosine kinase receptor inhibitor that has been confirmed to exert inhibitory effect on the platelet derived growth factor PDGF receptor (PDGFRα and PDGFRβ) activity. OBJECTIVE: To investigate the protective effect of imatinib on the myocardial fibrosis in deoxycorticosterone-acetate (DOCA)/salt induced hypertensive rats. METHODS: Sixty male uninephrectomized Sprague-Dawley rats were assigned to three groups: control rats (CON group); deoxycorticosterone group (DOCA group); deoxycorticosterone and imatinib group (DOCA+IMA group). Systolic blood pressure (SBP) was measured biweekly. The apical portion of the left ventricle was studied. Sirius-Red staining, Hematoxylin-Eosin staining, immunohistochemistry and Western blot assay were employed. RESULTS: SBP in the DOCA group and DOCA+IMA group was higher than that in the CON group on day 14 and 28. Animals in the DOCA group showed severe interstitial and perivascular fibrosis on day 28, and the expressions of PI, PIII, tenascin-C and fibronectin were significantly higher than those in the DOCA+IMA group and CON group. When compared with the CON group, myocardial tissue inflammatory response and monocyte/macrophage infiltration of different degrees were observed in the DOCA group and DOCA+IMA group. Protein expressions of PDGF-A, PDGF-C and PDGFRα were signiflcantly higher in the DOCA and DOCA+IMA groups than those in the CON group, but the p-PDGFRα protein expression in the DOCA+IMA group was lower than that in the DOCA group. CONCLUSION: Imatinib can exert inhibitory effects on myocardial fibrosis in DOCA/salt induced hypertensive rats, which may be attributed to the inhibition of PDGFR-α activity.

Dois novos diterpenos com esqueleto caurano, 3a,16b-17-tri-hidróxi-ent-caurano 3-O-β-D-glucopiranosídeo e ácido 2β,3α-di-hidróxi-ent-caur-15-eno-17-óico 3-O-β-D-glucopiranosídeo, foram isolados das cascas de Albizia mollis (Wall.) Boiv. As estruturas destes dois cauranos foram elucidadas por métodos espectroscópicos uni e bidimensionais e por espectrometria de massas.

Two new kaurane diterpenoids, 3α, 16β, 17-trihydroxy-ent-kaurane 3-O-β-D-glucopyranoside and 2β,3α-dihydroxy-ent-kaur-15-en-17-oic acid 3-O-β-D-glucopyranoside, were isolated from the bark of Albizia mollis (Wall.) Boiv. The structures of two new compounds were elucidated by extensive 1D- and 2D-NMR spectroscopic methods in combination with MS experiments.

Glycosides are the bioactive components of many famous Chinese medicines. Here reported are some bioactive glycosides we discovered from Chinese medicines in recent years. (1) Pheolic glycosides from Chinese medicines: Gastrodia elata, acontium austroynanense and Helicia erratica, three bioactive phenolic glycosides were discovered and two of them have been developed into new drugs. (2) Terpenoidal glycosides: a) Monoterpenoid: the sweroside from Swertia mollensis has been developed intro an anti-hepatitis drug; b) Diterpenoid: Phlomis betonicoides contains sweet glycoides; c) Triterpenoid: many biologically active triterpenoid glycosides were isolated from Panax plants and Siraitia grosvenorii. (3) Steroidal glycosides: a) C21-steroid: Cynanchum otophyllum and C. atratrum contain anti-epilepsy and-tumor glycosides; b) C27-steroid Hemostatic saponins were found in Paris polyphylla.